Modeling human disease

crispr eyes

In collaboration with the laboratories of Kate O’Connor-Giles  and Jill Wildonger, we developed Cas9-mediated genome editing in Drosophila. Since the original publication, we have continued to use and develop this facile genome engineering technology to probe the regulation of gene expression and to model human disease.



Figure caption:

The CRISPR-Cas9 system efficiently edits the Drosophila genome. Fluorescent markers indicate Cas9-mediated genome editing events. Photo credit: Scott Gratz.

Related publications:

Jain, S.U., Rashoff, A.Q., Krabbenhoft, S.D., Hoelper, D., Do, T.J., Gibson, T.J., Lundgren, S.M., Bondra, E.R., Deshmukh, S., Harutyunyan, A.S., Juretic, N., Jabado, N., Harrison, M.M., Lewis, P.W. (2020) H3 K27M and EZHIP Impede H3K27-Methylation Spreading by Inhibiting Allosterically Stimulated PRC2. Mol Cell. 80:726-735.

McDaniel, S.L., Hollatz, A.J., Branstad, A.M., Gaskill, M.M., Fox, C.A., and Harrison, M.M. (2020). Tissue-Specific DNA Replication Defects in Drosophila melanogaster Caused by a Meier-Gorlin Syndrom Mutation in Orc4. Genetics. 214:355-367.

Bier, E., Harrison, M.M., O’Connor-Giles, K.M., and Wildonger, J. (2018). Advances in Engineering the Fly Genome with the CRISPR-Cas System. Genetics 1:1-18.

Hamm, D.C., Larson, E.D., Nevil, M. Marshall, K.E., Bondra, E.R., and Harrison, M.M. (2017). A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster. PLoS Genet 13:e1007120.

Gratz, S.J., Rubinstein, D., Harrison, M.M., Wildonger, J., and K.M. O’Connor-Giles. (2015) CRISPR/Cas9- genome editing in Drosophila. Curr Protoc Mol Biol. 111: 31.2.1 -31.2.20.

Gratz, S.J., Harrison, M.M., Wildonger, J., and K.M. O’Connor-Giles. (2015) CRISPR/Cas9- genome editing in Drosophila. Methods Mol Biol. 1311: 335-348.

Harrison, M.M., Jenkins, B.V., O’Connor-Giles, K.M. and J. Wildonger. (2014) A CRISPR view of development. Genes Dev. 28:1859-1872.

Gratz, S.J., Wildonger, J., Harrison, M.M., and K.M. O’Connor-Giles. (2013) CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly. 7:249-255.

Gratz, S.J., Cummings, A.M., Nguyen, J.N., Hamm, D.C., Donohue, L.K., Harrison, M.M., Wildonger, J. and K.M. O’Connor-Giles. (2013) Genome engineering of Drosophila with the CRISPR RNA-guided Cas9-nuclease. Genetics. 194:1029-1035.

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